Cryo bio-crystallography: Difference between revisions
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==Basic principles== |
==Basic principles== |
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Cryo crystallography enables [[X-ray]] data collection at cryogenic |
Cryo crystallography enables [[X-ray]] data collection at cryogenic temperatures, typically 100K. |
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#Crystals are transferred from the solution they have grown in (called mother liquor) to a solution with a cryo-protectant to prevent ice formation. |
#Crystals are transferred from the solution they have grown in (called mother liquor) to a solution with a cryo-protectant to prevent ice formation. |
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Revision as of 11:17, 8 February 2020
Cryo bio-crystallography is the application of crystallography to biological macromolecules at cryogenic temperatures.
Basic principles
Cryo crystallography enables X-ray data collection at cryogenic temperatures, typically 100K.
- Crystals are transferred from the solution they have grown in (called mother liquor) to a solution with a cryo-protectant to prevent ice formation.
- Crystals are mounted with a glass fiber (as opposed to a capillary)
- Crystals are cooled by dipping directly into liquid nitrogen and then placed in a cryo cold stream.
- Cryo cooled macromolecular crystals show reduced radiation damage by more than 70 times that at room temperature.
Advantages
- Significant improvement of resolution in data collection
- Reduced or eliminated radiation damage in crystals
Usefulness and applications
Crystallography of large biological macromolecules can be achieved while maintaining their solution state. The most known example is the ribosome.[1]
References
- ^ Hope H (1988). "Cryocrystallography of biological macromolecules: a generally applicable method". Acta Crystallogr. B. 44 (1): 22–26. doi:10.1107/s0108768187008632. PMID 3271102.