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{{Short description|Species of bacterium}}
{{Short description|Species of bacterium}}
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'''Applications:'''
'''Applications:'''


Modified '''''Nostoc punctiforme''''' [[intein]] (Npu DnaE split [[intein]]) is used for a self-cleaving [[protein purification]] (e.g. ''i''CapTag™).<ref>{{Cite journal |last1=Cooper |first1=Merideth A. |last2=Taris |first2=Joseph E. |last3=Shi |first3=Changhua |last4=Wood |first4=David W. |date=2018 |title=A Convenient Split‐Intein Tag Method for the Purification of Tagless Target Proteins |url=https://onlinelibrary.wiley.com/doi/10.1002/cpps.46 |journal=Current Protocols in Protein Science |language=en |volume=91 |issue=1 |pages=5.29.1–5.29.23 |doi=10.1002/cpps.46 |pmid=29516483 |s2cid=3749506 |issn=1934-3655}}</ref><ref>{{Cite journal |last1=Prabhala |first1=Sai Vivek |last2=Gierach |first2=Izabela |last3=Wood |first3=David W. |date=2022 |title=The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History |url=https://www.frontiersin.org/article/10.3389/fmolb.2022.857566 |journal=Frontiers in Molecular Biosciences |volume=9 |page=857566 |doi=10.3389/fmolb.2022.857566 |doi-broken-date=31 December 2022 |pmid=35463948 |pmc=9033041 |issn=2296-889X}}</ref>
Modified '''''Nostoc punctiforme''''' [[intein]] (Npu DnaE split [[intein]]) is used for a self-cleaving [[protein purification]] (e.g. ''i''CapTag™).<ref>{{Cite journal |last1=Cooper |first1=Merideth A. |last2=Taris |first2=Joseph E. |last3=Shi |first3=Changhua |last4=Wood |first4=David W. |date=2018 |title=A Convenient Split‐Intein Tag Method for the Purification of Tagless Target Proteins |url=https://onlinelibrary.wiley.com/doi/10.1002/cpps.46 |journal=Current Protocols in Protein Science |language=en |volume=91 |issue=1 |pages=5.29.1–5.29.23 |doi=10.1002/cpps.46 |pmid=29516483 |s2cid=3749506 |issn=1934-3655}}</ref><ref>{{Cite journal |last1=Prabhala |first1=Sai Vivek |last2=Gierach |first2=Izabela |last3=Wood |first3=David W. |date=2022 |title=The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History |journal=Frontiers in Molecular Biosciences |volume=9 |page=857566 |doi=10.3389/fmolb.2022.857566 |pmid=35463948 |pmc=9033041 |issn=2296-889X|doi-access=free }}</ref>


== References ==
== References ==

Latest revision as of 03:10, 30 October 2023

Nostoc punctiforme
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Cyanobacteria
Class: Cyanophyceae
Order: Nostocales
Family: Nostocaceae
Genus: Nostoc
Species:
N. punctiforme
Binomial name
Nostoc punctiforme
(Kütz.) Har.

Nostoc punctiforme is a species of filamentous cyanobacterium. Under non-limiting nutritional environmental conditions, its filaments are composed of photosynthetic vegetative cells; upon nutrient limitation, some of these cells undergo differentiation into heterocysts, akinetes or hormogonia.[1]

N. punctiforme is one of the Nostoc strains able to maintain diazotrophic symbiosis with higher plants such as the bryophytes Anthocerus punctatus and Blasia pusilla, water ferns from the genus Azolla, the cycads Macrozamia spp., and the angiosperm Gunnera.[2]


Applications:

Modified Nostoc punctiforme intein (Npu DnaE split intein) is used for a self-cleaving protein purification (e.g. iCapTag™).[3][4]

References

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  1. ^ Meeks, JC; EL Campbell; ML Summers; FC Wong (2002). "Cellular differentiation in the cyanobacterium Nostoc punctiforme". Arch Microbiol. 178 (6): 395–403. doi:10.1007/s00203-002-0476-5. PMID 12420158. S2CID 17609733.
  2. ^ Meeks, JC (1998). "Symbiosis between Nitrogen-Fixing Cyanobacteria and Plants. They grow with the help of water". BioScience. 48 (4): 266–276. doi:10.2307/1313353. JSTOR 1313353.
  3. ^ Cooper, Merideth A.; Taris, Joseph E.; Shi, Changhua; Wood, David W. (2018). "A Convenient Split‐Intein Tag Method for the Purification of Tagless Target Proteins". Current Protocols in Protein Science. 91 (1): 5.29.1–5.29.23. doi:10.1002/cpps.46. ISSN 1934-3655. PMID 29516483. S2CID 3749506.
  4. ^ Prabhala, Sai Vivek; Gierach, Izabela; Wood, David W. (2022). "The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History". Frontiers in Molecular Biosciences. 9: 857566. doi:10.3389/fmolb.2022.857566. ISSN 2296-889X. PMC 9033041. PMID 35463948.
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