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{{Short description|Class of enzymes}}
{{Infobox enzyme
{{Infobox enzyme
| Name = Ent-kaurenoic acid oxidase
| Name = Ent-kaurenoic acid oxidase
| EC_number = 1.14.13.79
| EC_number = 1.14.13.79
| CAS_number = 337507-95-6
| CAS_number = 337507-95-6
| GO_code =
| IUBMB_EC_number = 1/14/13/79
| GO_code =
| image =
| image =
| width =
| width =
| caption =
| caption =
}}
}}
'''Ent-kaurenoic acid oxidase''' ({{EC number|1.14.13.79}}) is an [[enzyme]] with system name ''ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating)''.<ref>{{cite journal | title = The CYP88A cytochrome P450, <em>ent</em>-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway |author = Helliwell, C.A., Chandler, P.M., Poole, A., Dennis, E.S. and Peacock, W.J. |journal = Proc. Natl. Acad. Sci. USA |year = 2001 |volume = 98 |pages = 2065–2070 |pmid = 11172076}}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]
'''Ent-kaurenoic acid oxidase''' ({{EC number|1.14.13.79}}) is an [[enzyme]] with [[List of enzymes|systematic name]] ''ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating)''.<ref>{{cite journal | vauthors = Helliwell CA, Chandler PM, Poole A, Dennis ES, Peacock WJ | title = The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 98 | issue = 4 | pages = 2065–70 | date = February 2001 | pmid = 11172076 | pmc = 29382 | doi = 10.1073/pnas.041588998 | doi-access = free }}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]


: ent-kaur-16-en-19-oate + 3 NADPH + 3 H<sup>+</sup> + 3 O<sub>2</sub> <math>\rightleftharpoons</math> gibberellin A12 + 3 NADP<sup>+</sup> + 4 H<sub>2</sub>O (overall reaction)
: ent-kaur-16-en-19-oate + 3 NADPH + 3 H<sup>+</sup> + 3 O<sub>2</sub> <math>\rightleftharpoons</math> gibberellin A12 + 3 NADP<sup>+</sup> + 4 H<sub>2</sub>O (overall reaction)
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== External links ==
== External links ==
* {{MeshName|Ent-kaurenoic+acid+oxidase}}
* {{MeshName|Ent-kaurenoic+acid+oxidase}}

{{Dioxygenases}}
{{Enzymes}}
{{Portal bar|Biology|border=no}}


[[Category:EC 1.14.13]]
[[Category:EC 1.14.13]]


{{Enzyme-stub}}

Latest revision as of 13:58, 26 August 2023

Ent-kaurenoic acid oxidase
Identifiers
EC no.1.14.13.79
CAS no.337507-95-6
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

Ent-kaurenoic acid oxidase (EC 1.14.13.79) is an enzyme with systematic name ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating).[1] This enzyme catalyses the following chemical reaction

ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2 gibberellin A12 + 3 NADP+ + 4 H2O (overall reaction)
(1a) ent-kaur-16-en-19-oate + NADPH + H+ + O2 ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
(1b) ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2 gibberellin A12 aldehyde + NADP+ + 2 H2O
(1c) gibberellin A12 aldehyde + NADPH + H+ + O2 gibberellin A12 + NADP+ + H2O

Ent-kaurenoic acid oxidase requires cytochrome P450.

References

[edit]
  1. ^ Helliwell CA, Chandler PM, Poole A, Dennis ES, Peacock WJ (February 2001). "The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway". Proceedings of the National Academy of Sciences of the United States of America. 98 (4): 2065–70. doi:10.1073/pnas.041588998. PMC 29382. PMID 11172076.
[edit]